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pucD [2019-08-09 10:06:44]
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pucD [2019-08-09 10:06:44]

xanthine dehydrogenase
Locus
BSU_32480
Isoelectric point
5.75
Molecular weight
80.25 kDa
Protein length
745 aa Sequence Blast
Gene length
2238 bp Sequence Blast
Function
purine utilization
Product
xanthine dehydrogenase
Essential
no
Synonyms
yurC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Utilization of nucleotides

    • Gene

    Coordinates
    3,336,263 → 3,338,500

    The protein

    Catalyzed reaction/ biological activity

  • H2O + NAD+ + xanthine --> H+ + NADH + urate (according to UniProt)

    • Protein family

  • xanthine dehydrogenase family (single member, according to UniProt)

    • Structure

  • 1RM6 (4-hydroxybenzoyl-CoA reductase from Thauera aromatica, 30% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    pucA-pucB-pucC-pucD-pucE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • PucR: activation, PubMed, in PucR regulon

    • Regulation

  • induced in the presence of purine nucleotides (effector: allantoin) (PucR) PubMed
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    Biological materials

    Mutant

  • MGNA-A964 (yurC::erm), available at the NBRP B. subtilis, Japan
  • BKE32480 (ΔpucD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATTCCCCTCCCTCAG, downstream forward: _UP4_CAATGGACATAAAAGAGGCC
  • BKK32480 (ΔpucD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATTCCCCTCCCTCAG, downstream forward: _UP4_CAATGGACATAAAAGAGGCC

    • References

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