mutY [2019-07-25 10:02:40]

A/G-specific adenine glycosylase, error prevention oxidized guanine system, releases adenines from 8-oxo-G:A mismatches

Locus: BSU_08630

Isoelectric point: 6.04

Molecular weight: 41.81 kDa

Protein length: 369 aa

Gene length: 1110 bp

Function: DNA repair

Product: A/G-specific adenine glycosylase

Essential: no

E.C.: 3.2.2.-

Synonyms: yfhQ

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Oxidized guanine (GO) DNA repair system

Gene

Coordinates: 935,656 → 936,765

Phenotypes of a mutant

• strongly reduced stationary phase mutagenesis PubMed
• increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage PubMed

The protein

Catalyzed reaction/ biological activity

• releases adenines from 8-oxo-G:A mismatches

Protein family

• Nth/MutY family (with Nth, according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 1RRS (complex with DNA, Geobacillus stearothermophilus)

Expression and Regulation

Operons

• Genes: mutY-fabL-sspE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigG: sigma factor, PubMed, in SigG regulon

  Regulatory mechanism

  • YfhP: repression, in YfhP regulon

  Regulation

  • expression is constitutive throughout growth PubMed

Biological materials

Mutant

• MGNA-C322 (yfhQ::erm), available at the NBRP B. subtilis, Japan
• BKE08630 (ΔmutY::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTGTTTCTCCCCAGT, downstream forward: _UP4_ATCTCGGCTGCTCCGTAAAC
• BKK08630 (ΔmutY::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTGTTTCTCCCCAGT, downstream forward: _UP4_ATCTCGGCTGCTCCGTAAAC

References

Reviews

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Original publications

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