rex

transcriptional repressor of anaerobically expressed genes involved in anaerobic respiration and fermentation

locus: BSU_05970

pI: 6.77

mw: 23.95 kDa

protein length: 215 aa

gene length: 648 bp

function: regulation of fermentation and anaerobic respiration

product: transcriptional repressor

essential: no

synonyms: ydiH

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.1|Regulators of electron transport]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 647,091 → 647,738

Phenotypes of a mutant

• inactivation of ''[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]'' reduces sporulation efficiency to 9.2% that of wild type cells [Pubmed|26735940]
• inactivation of ''[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]'' confers resistance to high concentrations of Zn(II) [Pubmed|27935957]

The protein

Protein family

• transcriptional regulatory rex family (single member, according to UniProt)

Effectors of protein activity

• NADH (indication of low oxygen concentration) releases Rex from DNA [Pubmed|18485070], Rex has a very high affinity for NADH

Structure

• [PDB|2VT2], [PDB|2VT3]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000176 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]-[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]-[gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]
  description: [Pubmed|22383849]

  regulation

  • constitutitvely expressed [Pubmed|22383849]
  • the mRNA is processed between [gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex] and [gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

Mutant

• MGNA-C201 (ydiH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2199 NBRP B. subtilis, Japan]
• LUW292 (erm), GP834 (erm), both available in the [SW|Stülke] lab
• BKE05970 (Δ[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGGAATTTTTGATTGATCCT, downstream forward: _UP4_TAGAGGGAAAGGAGGAGCCC
• BKK05970 (Δ[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGGAATTTTTGATTGATCCT, downstream forward: _UP4_TAGAGGGAAAGGAGGAGCCC

labs

• [SW|Claes von Wachenfeldt], Lund University, Sweden [http://aron.ldc.lu.se/kundwebb/cellorg/mibiol/research/wachen/index.htm Homepage]

References

Reviews

• 23046954

Original publications

• 16207915,18485070,15231791,17015645,20525796,21402078,22383849,25216269,26735940,27935957,29794222