SubtiBank SubtiBank
ptsG [Thu Jan 07 2016 20:01:33 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ptsG [Thu Jan 07 2016 20:01:33 GMT+0100 (CET)]

trigger enzyme: major glucose permease of the PTS, EIICBA(Glc) and control of GlcT activity
locus
BSU13890
pI
5.00
mw
75.00 kDa
function
glucose transport and phosphorylation, control of GlcT activity
product
trigger enzyme: glucose-specific enzyme IICBA component of the PTS
essential
no
ec
2.7.1.69
synonyms
ptsX, crr
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • [SW|Categories] containing this gene/protein

  • [SW|phosphotransferase systems], [SW|carbon core metabolism], [SW|transcription factors and their control], [SW|trigger enzyme], [SW|membrane proteins], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|GlcT regulon], [SW|stringent response]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,457,187 -> 1,459,286

    The protein

    Catalyzed reaction/ biological activity

  • transport and phosphorylation of glucose, receives a phosphate from [PtsH|search|HPr] at the IIA domain (His-620), the phosphate group is then transferred to the IIB domain (Cys-461) an finally to the incoming glucose. In the absence of glucose, PtsG phosphorylates and thereby inactivates the transcriptional antiterminator [protein|search|GlcT].

    • Protein family

  • [SW|PTS] permease, glucose permease (Glc) family [Pubmed|10627040], [SW|PTS] enzyme II

    • [SW|Domains]

  • 11x transmembrane domain (16–36, 89–109, 139–159, 180–200, 233–253, 283–303, 313–333, 338–358, 365–385, 388–408)
  • PTS EIIC domain ( 1-424)
  • PTS EIIB domain (439–520)
  • PTS EIIA domain (568–672)

    • Modification

  • transient phosphorylation ([PtsH|search|HPr]-dependent) on His-620, then internal phosphotransfer from His-620 to Cys-461

    • Structure

  • [PDB|1AX3] (IIA domain), [PDB|1GPR] (IIA domain), IIA domain [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=56258 NCBI], NMR IIA domain [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=55395 NCBI]

    • [SW|Localization]

  • membrane protein [Pubmed|18763711]

    • [SW|Interactions]

  • [PtsH|search|HPr]-[protein|search|PtsG] [Pubmed|8418852]
  • PtsG-[protein|search|GlcT] (for phosphorylation of [protein|search|GlcT]) [Pubmed|14527945]

    • Expression and Regulation

    Operon

  • ''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' [Pubmed|11902727]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11902727]

    • Regulation

  • expression activated by glucose (32 fold) ([protein|search|GlcT]) [Pubmed|12850135]
  • subject to negative stringent control upon lysine starvation [Pubmed|20081037]

    • Regulatory mechanism

  • transcriptional antitermination via the [protein|search|GlcT]-dependent [SW|RNA switch] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 9765562 PubMed]
  • stringent response: due to presence of guanine at 1 position of the transcript [Pubmed|20081037]

    • Biological materials

    Expression vector

  • pGP123 (domains BA, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP141 (domains BA, mut: H620D, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP428 (EIIB, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP437(EIIA in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP34 ([protein|search|pAC5]) [Pubmed|11902727], available in [SW|Jörg Stülke]'s lab
  • pGP66 ([protein|search|pAC7]) [Pubmed|11902727], available in [SW|Jörg Stülke]'s lab
  • pGP606 (mutant terminator, [protein|search|pAC6]), available in [SW|Jörg Stülke]'s lab
  • pGP532 ([protein|search|pAC7]), available in [SW| Jörg Stülke]'s lab
  • series of promoter deletions are available in [protein|search|pAC5] and [protein|search|pAC6], available in [SW|Jörg Stülke]'s lab
  • series of RAT mutants are available in [protein|search|pAC6], available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

    • References

    Reviews

  • 18086213

    • Original publications

  • 10627040,12850135,18763711,11902727,9765562,9513271,1956301,10543968,17074746,15155854,14527945,1508157,2120236,9593197,8418852,1581296,1316146,1733770,1942043,1911744,1906345,20081037,22846916