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ptsG [2018-02-23 18:52:10]
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ptsG [2018-02-23 18:52:10]

trigger enzyme
Locus
BSU13890
Isoelectric point
5.41
Molecular weight
75.34 kDa
Protein length
699 aa Sequence Blast
Gene length
2097 bp Sequence Blast
Function
glucose transport and phosphorylation, control of GlcT activity
Product
trigger enzyme
Essential
no
E.C.
2.7.1.69
Synonyms
ptsX,crr
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Carbon core metabolism → Glycolysis
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of PRD-type regulators
  • Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue

    • Gene

    Coordinates
    1,457,187 → 1,459,286

    The protein

    Catalyzed reaction/ biological activity

  • transport and phosphorylation of glucose, receives a phosphate from HPr]] at the IIA domain (His-620), the phosphate group is then transferred to the IIB domain (Cys-461) an finally to the incoming glucose. In the absence of glucose, PtsG phosphorylates and thereby inactivates the transcriptional antiterminator GlcT.

    • Protein family

  • PtsI permease, glucose permease (Glc) family PubMed, PtsI enzyme II

    • Domains

  • 11x transmembrane domain (16–36, 89–109, 139–159, 180–200, 233–253, 283–303, 313–333, 338–358, 365–385, 388–408)
  • PTS EIIC domain ( 1-424)
  • PTS EIIB domain (439–520)
  • PTS EIIA domain (568–672)

    • Modification

  • transient phosphorylation (HPr]]-dependent) on His-620, then internal phosphotransfer from His-620 to Cys-461

    • Structure

  • 5IWS (the IIC domain of E. coli MalT, 32% identity)
  • 1AX3 (IIA domain)
  • 1GPR (IIA domain)

    • Localization

  • membrane protein PubMed

    • Expression and Regulation

    Operons

    Genes
    ptsG-ptsH-ptsI
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • GlcT: antitermination, via the GlcT-dependent RNA switch PubMed, in GlcT regulon

    • Regulation

  • expression activated by glucose (2 fold) (GlcT) PubMed
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    Biological materials

    Mutant

  • BKE13890 (ΔptsG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGAATTGACCTCCTCT, downstream forward: _UP4_TAAGGGTGTTAGTACGCCGT
  • BKK13890 (ΔptsG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGAATTGACCTCCTCT, downstream forward: _UP4_TAAGGGTGTTAGTACGCCGT

    • Expression vector

  • pGP123 (domains BA, in pWH844), available in Jörg Stülke's lab
  • pGP141 (domains BA, mut: H620D, in pWH844), available in Jörg Stülke's lab
  • pGP428 (EIIB, in pWH844), available in Jörg Stülke's lab
  • pGP437(EIIA in pGP570, with thrombin cleavage site), available in Jörg Stülke's lab

    • LacZ fusion

  • pGP34 (pAC5) PubMed, available in Jörg Stülke's lab
  • pGP66 (pAC7) PubMed, available in Jörg Stülke's lab
  • pGP606 (mutant terminator, pAC6), available in Jörg Stülke's lab
  • pGP532 (pAC7), available in Jörg Stülke's lab
  • series of promoter deletions are available in pAC5 and pAC6, available in Jörg Stülke's lab
  • series of RAT mutants are available in pAC6, available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Jörg Stülke, University of Göttingen, Germany
  • Homepage

    • References

    Reviews

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    Original publications

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