thiG [2017-8-11 16:0:3]

hydroxyethylthiazole phosphate biosynthesis

locus: BSU11690

pI: 4.72

mw: 26.87 kDa

protein length: 256 aa

gene length: 768 bp

function: biosynthesis of thiamine

product: thiamin thiazole synthase

essential: no

synonyms: yjbT

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 1,245,041 → 1,245,811

The protein

Protein family

• thiG family (according to Swiss-Prot)

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]

Structure

• [PDB|1XM3], [PDB|1TYG] (complex with [protein|95DA3F899037140F48B99E065C768356CE08FA94|ThiS]) [Pubmed|15362849]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]
  description: [Pubmed|1898926]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

  regulation

  • repressed by thiamine ([SW|Thi-box]) [Pubmed|16356850]
  • the [SW|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B165 (yjbT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1164 NBRP B. subtilis, Japan]

References

Reviews

• 19348578,10382260

Original publications

• 15362849,17726680,16493705,19216519,14567704