fadE

acyl-CoA dehydrogenase

Locus: BSU_32820

Isoelectric point: 5.15

Molecular weight: 65.16 kDa

Protein length: 594 aa

Gene length: 1785 bp

Function: fatty acid degradation

Product: acyl-CoA dehydrogenase

Essential: no

Synonyms: yusJ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,367,040 → 3,368,824

The protein

Catalyzed reaction/ biological activity

• A + 2,3-saturated acyl-CoA --> 2,3-dehydroacyl-CoA + AH₂ (according to UniProt)

Protein family

• Acyl-CoA dehydrogenase family (according to UniProt)

Paralogous protein(s)

• YngJ, AcdA
• MmgC: (40.2%)

Cofactors

• FAD (according to UniProt)

Structure

• 2Z1Q (from Thermus thermophilus hb8, 52% identity, 68% similarity)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: fadN-fadA-fadE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • SdpR: activation, PubMed, in SdpR regulon
  • FadR: repression, PubMed, in FadR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • FadN: induced by long-chain fatty acids (FadR) PubMed
  • expression of the operon is induced during biofilm formation PubMed

• Genes: fadM-fadN-fadA-fadE
  Description: PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon
  • SdpR: activation, PubMed, in SdpR regulon

  Regulation

  • fadM: induced by long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

• MGNA-A602 (yusJ::erm), available at the NBRP B. subtilis, Japan
• BKE32820 (fadE::erm, available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed
• BKE32820 (ΔfadE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGTTTTTCCCCCTTTA, downstream forward: _UP4_TGATTGGCCATGAGCTCCGC
• BKK32820 (ΔfadE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGTTTTTCCCCCTTTA, downstream forward: _UP4_TGATTGGCCATGAGCTCCGC

References

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