ansZ [Tue Nov 01 2016 10:33:14 GMT+0100 (CET)]

asparaginase

locus: BSU02690

pI: 6.00

mw: 39.00 kDa

function: asparagine utilization

product: asparaginase

essential: no

ec: 3.5.1.1

synonyms: yccC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.6|Utilization of asparagine/ aspartate]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|CodY regulon], [SW|TnrA regulon]

Gene

Coordinates on the chromosome (coding sequence): 290,915 -> 292,042

The protein

Catalyzed reaction/ biological activity

• L-asparagine H₂O = L-aspartate NH₃ (according to Swiss-Prot)

Protein family

• asparaginase 1 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|AnsA]

Structure

• [PDB|1ZCF] (from ''Pectobacterium atrosepticum scri1043'', 55% identity, 73% similarity)

[SW|Interactions]

• forms tetramers

Expression and Regulation

Operon

• ''ansZ'' [Pubmed|11914346]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|11914346]

Regulation

• expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|11914346]
• repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]

Regulatory mechanism

• [protein|search|TnrA]: transcription activation [Pubmed|11914346]
• [protein|search|CodY]: transcription repression [Pubmed|12618455]

References

• 24003863,11914346,12618455,26672444