function
production of nitric oxide
product
nitric-oxide synthase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds][SW|Categories] containing this gene/protein
[SW|miscellaneous metabolic pathways], [SW|biosynthesis of antibacterial compounds]Gene
Coordinates on the chromosome (coding sequence)
836,653 -> 837,744
The protein
Catalyzed reaction/ biological activity
NADPH-dependent oxidation of l-arginine into l-citrulline [Pubmed|25826316]Protein family
Bacterial NOS oxygenase subfamily (according to Swiss-Prot)[SW|Cofactors]
heme b [Pubmed|20006999]NADPH [Pubmed|25826316]FMN or FAD [Pubmed|21635694]Structure
[PDB|2AMO] (loose dimer), [PDB|1M7V] (bound to tetrahydrofolate and arginine)[SW|Interactions]
[protein|search|Nos]-[protein|search|YkuN] (electron transfer from to [protein|search|Nos]) [Pubmed|25194416]Expression and Regulation
Operon
''[protein|search|nos]-[protein|search|yflK]'' (according to [http://dbtbs.hgc.jp/COG/prom/yflMK.html DBTBS])Additional information
Note that the gene '[protein|search|yflL]' is located between '[protein|search|nos]' and '[protein|search|yflK]', but on the opposite strand.Biological materials
Mutant
MGNA-C254 (yflM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2252 NBRP B. subtilis, Japan]References
Reviews
Original publications
12220171,11856757,20006999,21599925,22119809,23943262,21310962,21921039,25194416,25826316,26062720 
