ndk

nucleoside diphosphate kinase

Locus: BSU_22730

Isoelectric point: 5.57

Molecular weight: 16.82 kDa

Protein length: 149 aa

Gene length: 450 bp

Function: maintainance of an equilibrium between the concentrations of different nucleoside triphosphates

Product: nucleoside diphosphate kinase

Essential: no

E.C.: 2.7.4.6

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Nucleotide metabolism/ other
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

Gene

Coordinates: 2,381,354 → 2,381,803

Phenotypes of a mutant

• inactivation of ndk reduces sporulation efficiency to 13.4% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• 2'-deoxyribonucleoside 5'-diphosphate + ATP --> 2'-deoxyribonucleoside 5'-triphosphate + ADP (according to UniProt)
• ribonucleoside 5'-diphosphate + ATP --> ribonucleoside 5'-triphosphate + ADP (according to UniProt)

Protein family

• NDK family (single member, according to UniProt)

Modification

• phosphorylation on Thr-92 AND Ser-123 PubMed

Structure

• 1WKJ (from Thermus thermophilus, 59% identity, 82% similarity)

Expression and Regulation

Operons

• Genes: hepS-menH-hepT-ndk
  Description: PubMed

• Genes: ndk
  Description: PubMed

Biological materials

Mutant

• BKE22730 (Δndk::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCTCCACCCCATCAT, downstream forward: _UP4_TAATCATTGTGAAAATAAGC
• BKK22730 (Δndk::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCTCCACCCCATCAT, downstream forward: _UP4_TAATCATTGTGAAAATAAGC

References

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