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ytoI [2019-08-28 09:03:53]
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ytoI [2019-08-28 09:03:53]

similar to transcriptional regulator ([SW|GntR family])
locus
BSU_29270
pI
6.59
mw
48.87 kDa
protein length
439 aa Sequence Blast
gene length
1320 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]

    • Gene

    Coordinates
    2,997,301 → 2,998,620

    Phenotypes of a mutant

  • an ''ytoI'' point mutation has been isolated upon selction at low pressure [Pubmed|26296725]

    • The protein

    Protein family

  • [SW|GntR family] of transcription factors

    • [SW|Domains]

  • 2 [SW|CBS domain]s (195-254, 256-314)

    • Expression and Regulation

    Operons

    genes
    [gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]
    description
    [Pubmed|9387221]

    additional information

  • An [ncRNA|search|antisense RNA] is predicted for'[protein|search|ytoI]' [PubMed|20525796]
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    Biological materials

    Mutant

  • MGNA-A152 (ytoI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/152 NBRP B. subtilis, Japan]
  • GP2213 (''[gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • GP2214 (''[gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]''::''phleo''), available in [SW|Jörg Stülke]'s lab
  • BKE29270 (Δ[gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCCTTTCACCTCTAAGG, downstream forward: _UP4_AGCTAAGGGACGTTCAGGCG
  • BKK29270 (Δ[gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCCTTTCACCTCTAAGG, downstream forward: _UP4_AGCTAAGGGACGTTCAGGCG

    • Expression vectors

  • pGP2933 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 16479537,9387221,20525796,23504016,26296725