ytoI

similar to transcriptional regulator (GntR family)

Locus: BSU_29270

Isoelectric point: 6.59

Molecular weight: 48.87 kDa

Protein length: 439 aa

Gene length: 1320 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factor/ other/ based on similarity

Gene

Coordinates: 2,997,301 → 2,998,620

Phenotypes of a mutant

• an ytoI point mutation has been isolated upon selction at low pressure PubMed

The protein

Protein family

• GntR family of transcription factors

Domains

• 2 CBS domains (aa 195-254, aa 256-314) (according to UniProt)

Expression and Regulation

Operons

• Genes: ytoI
  Description: PubMed

  Additional information

  • An [ncRNA|search|antisense RNA] is predicted for'ytoI' PubMed

Biological materials

Mutant

• MGNA-A152 (ytoI::erm), available at the NBRP B. subtilis, Japan
• GP2213 (ytoI::aphA3), available in Jörg Stülke's lab
• GP2214 (ytoI::phleo), available in Jörg Stülke's lab
• BKE29270 (ΔytoI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGCCTTTCACCTCTAAGG, downstream forward: _UP4_AGCTAAGGGACGTTCAGGCG
• BKK29270 (ΔytoI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGCCTTTCACCTCTAAGG, downstream forward: _UP4_AGCTAAGGGACGTTCAGGCG

Expression vectors

• pGP2933 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

References

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