tcyC

cystine and diaminopimelate ABC transporter, ATP-binding protein

Locus: BSU_03590

Isoelectric point: 7.19

Molecular weight: 27.64 kDa

Protein length: 247 aa

Gene length: 744 bp

Function: cystine and diaminopimelate uptake

Product: cystine and diaminopimelate ABC transporter, ATP-binding protein

Essential: no

Synonyms: yckI

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of amino acids
• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine
• Groups of genes → Membrane proteins

Gene

Coordinates: 409,208 → 409,951

The protein

Catalyzed reaction/ biological activity

• uptake of cystine and diaminopimelate PubMed

Protein family

• ABC transporter superfamily (according to UniProt)
• L-cystine importer (TC 3.A.1.3.14) family (single member, according to UniProt)

Paralogous protein(s)

• YxeO, PstBB, YvrO, GlnQ, ArtR, TcyN, PstBA

Domains

• ABC transporter domain (aa 2-240) (according to UniProt)

Structure

• 2Q0H (in complex with ADP-Mg2+, Geobacillus stearothermophilus, 55% identity)

Localization

• attached to the membrane via TcyB PubMed

Expression and Regulation

Operons

• Genes: tcyA-tcyB-tcyC
  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed

Biological materials

Mutant

• MGNA-C004 (yckI::erm), available at the NBRP B. subtilis, Japan
• BKE03590 (ΔtcyC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACGGTAAGCATACTCGGAA, downstream forward: _UP4_TAATAAGAAAAACAGAGCGT
• BKK03590 (ΔtcyC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACGGTAAGCATACTCGGAA, downstream forward: _UP4_TAATAAGAAAAACAGAGCGT

References

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