hbuT

sporulation protein, transporter for 3-hydroxybutyrate

locus: BSU_39000

pI: 8.90

mw: 49.98 kDa

protein length: 472 aa

gene length: 1419 bp

function: 3-hydroxybutyrate utilization

product: transporter for 3-hydroxybutyrate

essential: no

synonyms: yxjC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,002,716 → 4,004,134

Phenotypes of a mutant

• reduced uptake of 3-hydroxybutyrate during [SW|sporulation] [Pubmed|26363016]

The protein

Catalyzed reaction/ biological activity

• uptake of 3-hydroxybutyrate [Pubmed|26363016]

Protein family

• [SW|CitM transporter family (TC 2.A.11)] (according to UniProt)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]-[gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]-[gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]-[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135,10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|12662922]

Biological materials

Mutant

• MGNA-B727 (yxjC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1726 NBRP B. subtilis, Japan]
• BKE39000 (Δ[gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACACACATCCCCCGTTT, downstream forward: _UP4_TAGAAGAAAACGAAAGGGGC
• BKK39000 (Δ[gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACACACATCCCCCGTTT, downstream forward: _UP4_TAGAAGAAAACGAAAGGGGC

References

• 16672620,10746760,12662922,12850135,10666464,26363016