rodA [2019-07-15 10:02:52]

SEDS family peptidoglycan glycosyltransferase, control of cell shape and elongation

Locus: BSU_38120

Isoelectric point: 8.76

Molecular weight: 43.12 kDa

Protein length: 393 aa

Gene length: 1182 bp

Function: control of cell shape and elongation

Product: SEDS family peptidoglycan glycosyltransferase

Essential: yes

Synonyms: ywcF,ipa-42d

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell shape
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Groups of genes → Essential genes
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,912,332 → 3,913,513

Phenotypes of a mutant

• essential PubMed
• CRISPRi knockdown mutant has a terminal phenotype of severe lysis PubMed

The protein

Catalyzed reaction/ biological activity

• principal peptidoglycan glycosyltransferase of the elongasome PubMed

Protein family

• SEDS proteins (shape, elongation, division, and sporulation) PubMed

Paralogous protein(s)

• SpoVE, FtsW

Structure

• 6BAR (from Thermus thermophilus, 26% identity) PubMed

Localization

• integral membrane protein PubMed
• forms circumferentially moving patches PubMed
• localization depends on MreB 26679002

Expression and Regulation

Operons

• Genes: rodA
  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon

Biological materials

Mutant

• xylose-inducible CRISPRi knockdown mutant BEC38120 is available from the BGSC

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage
• David Rudner, Harvard Medical School, MA, USA homepage

References

Reviews

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Original Publications

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