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ykoV [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]
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ykoV [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

DNA-end-binding protein Ku, non-homologous end joining DNA repair
locus
BSU13410
pI
7.00
mw
34.00 kDa
function
non-homologous end joining DNA repair, repair of gapped DNA substrates
product
DNA-end-binding protein Ku
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.3|Spore-encoded non-homologous end joining system]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • [SW|Categories] containing this gene/protein

  • [SW|DNA repair/ recombination], [SW|sporulation proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|SigG regulon], [SW|SpoVT regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,406,357 -> 1,407,292

    Phenotypes of a mutant

  • sensitivity to ionizing radiation in the stationary phase [Pubmed|12215643]
  • sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat [Pubmed|16497325,17293412]
  • a ''[protein|search|ykoV]-[protein|search|ligD]'' double mutant is sensitive to radiation [Pubmed|24123749]

    • The protein

    Catalyzed reaction/ biological activity

  • stimulation of [SW|LigD] non-homologous end-joining activity [Pubmed|23691176,26961308]

    • [SW|Localization]

  • associates with the nucleoid during germination [Pubmed|16497325]

    • [SW|Interactions]

  • [protein|search|YkoV]-[protein|search|LigD], to stimulate [SW|LigD] activity [Pubmed|23691176,26961308]

    • Expression and Regulation

    Operon

  • ''[protein|search|ykoV]-[protein|search|ligD]'' [Pubmed|16497325]

    • [SW|Sigma factor]

  • [protein|search|SigG] [Pubmed|16497325]

    • Regulation

  • expressed during sporulation in the forespore ([protein|search|SigG], [SW|SpoVT]) [Pubmed|16497325]

    • Regulatory mechanism

  • [SW|SpoVT]: transcription activation [Pubmed|16497325]

    • Biological materials

    Mutant

  • BP141 (''[protein|search|ykoV]-[protein|search|ligD]''::''kan'') available in [SW|Fabian Commichau]'s lab
  • BP142 (''[protein|search|ykoW]-[protein|search|ykoV]-[protein|search|ligD]''::''kan'') available in [SW|Fabian Commichau]'s lab

    • References

    Reviews

  • 11591342,11719239,17938628,22933559

    • Original publications

  • 16497325,11483577,23691176,12215643,17293412,24123749,25355514,26961308