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putP [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
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putP [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

high affinity proline permease
locus
BSU03220
pI
8.00
mw
48.00 kDa
function
proline uptake
product
high affinity proline permease
essential
no
synonyms
ycgO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.1|Solute:sodium symporter family]
  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.7|Sodium-solute symporter]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.2|Utilization of proline]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|transporters/ other], [SW|biosynthesis/ acquisition of amino acids], [SW|utilization of amino acids], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|PutR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    347,150 -> 348,571

    Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen [Pubmed|21840319]

    • The protein

    Catalyzed reaction/ biological activity

  • uptake of proline coupled to the uptake of sodium ions

    • Protein family

  • sodium solute symporter family (SSS; TC2A.21)

    • Paralogous protein(s)

  • [protein|search|OpuE]

    • Structure

  • [PDB|2XQ2] (from ''Vibrio parahaemolyticus'', 26% identity) [Pubmed|21131949]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operon

  • ''[protein|search|putB]-[protein|search|putC]-[protein|search|putP]'' [Pubmed|21840319,22139509]
  • ''[protein|search|putP]'' (internal promoter) [Pubmed|21840319]

    • [SW|Sigma factor]

  • ''[protein|search|putB]'': [protein|search|SigA] [Pubmed|21840319,21964733,22139509]

    • Regulation

  • induced in the presence of proline ([protein|search|PutR]) [Pubmed|21840319,22139509]

    • Regulatory mechanism

  • [protein|search|PutR]: transcription activation [Pubmed|21840319,21964733,22139509]

    • Additional information

  • the mRNA is cleaved by [rnc|search|RNase III] [PubMed|26883633]

    • Biological materials

    Mutant

  • available in [SW|Erhard Bremer]'s lab [Pubmed|24142252]

    • References

  • 21840319,21964733,22139509,24142252,21131949,26728461,26883633