arsR

transcriptional regulator of the [SW|ArsR family], arsenic resistance operon repressor

locus: BSU_25810

pI: 4.88

mw: 12.15 kDa

protein length: 105 aa

gene length: 318 bp

function: regulation of As (III) efflux

product: transcription repressor ([SW|ArsR family])

essential: no

synonyms: yqcJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.11|Resistance against toxic metals]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.3|Skin element]

Gene

Coordinates: 2,657,317 → 2,657,634

The protein

Protein family

• [SW|ArsR family]

[SW|Domains]

• [SW|HTH arsR-type domain] (aa 4-99) (according to UniProt)

Structure

• [PDB|1U2W] (CadC from Staphylococcus aureus, 36% identity) [pubmed|15937183]

Expression and Regulation

Operons

• genes: [gene|B3D88B9C89154B77D77FB833CA16FD4F4E29FE96|arsR]-[gene|74B1AF103E6B236609CD184A1A409A9A04857181|yqcK]-[gene|B749D54249A28A471EF796903A34622A16D5B0BB|arsB]-[gene|DB3527FB78D0B7C144D1699D665788184F19AFE0|arsC]
  description: [Pubmed|9537360]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9537360], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulatory mechanism

  • [protein|B3D88B9C89154B77D77FB833CA16FD4F4E29FE96|ArsR]: repression, [Pubmed|9537360], in [regulon|B3D88B9C89154B77D77FB833CA16FD4F4E29FE96|ArsR regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325]

Biological materials

Mutant

• BKE25810 (Δ[gene|B3D88B9C89154B77D77FB833CA16FD4F4E29FE96|arsR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATATCGCCTTCTTTA, downstream forward: _UP4_TAAAAAAATTTTTTAGGTAT
• BKK25810 (Δ[gene|B3D88B9C89154B77D77FB833CA16FD4F4E29FE96|arsR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATATCGCCTTCTTTA, downstream forward: _UP4_TAAAAAAATTTTTTAGGTAT

References

• 15948947,9537360,16497325,15937183