SubtiBank SubtiBank
yqfL
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yqfL

Modulator of CcpN activity, similar to phosphoenolpyruvate synthetase regulatory protein
Locus
BSU_25240
Isoelectric point
5.78
Molecular weight
30.20 kDa
Protein length
270 aa Sequence Blast
Gene length
813 bp Sequence Blast
Function
inhibits CcpN activity
Product
modulator of CcpN activity
Essential
no
E.C.
2.7.-.-
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → Gluconeogenesis
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)

    • Gene

    Coordinates
    2,604,121 → 2,604,933

    The protein

    Catalyzed reaction/ biological activity

  • negative effector of CcpN activity PubMed
  • ADP + Nτ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] --> AMP + H+ + Nτ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)
  • H+ + Nτ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate --> diphosphate + Nτ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)

    • Protein family

  • pyruvate, phosphate/water dikinase regulatory protein family (single member, according to UniProt)

    • Domains

  • nucleotide binding domain (ATP) (151–158)

    • Structure

  • 5D0N (from maize, 39% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    ccpN-yqfL
    Description
    PubMed

    Regulation

  • constitutive PubMed

    • Additional information

  • the intracellular concentration of CcpN is about 4 myM (according to PubMed).
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    Biological materials

    Mutant

  • MGNA-C492 (yqfL::erm), available at the NBRP B. subtilis, Japan
  • BKE25240 (ΔyqfL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCC
  • BKK25240 (ΔyqfL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCC

    • Labs working on this gene/protein

  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

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