cotZ

spore crust anchor protein, necessary for the assembly of the spore crust, the outermost layer of the spore coat

Locus: BSU_11740

Isoelectric point: 5.00

Molecular weight: 16.00 kDa

Protein length: 148 aa

Gene length: 447 bp

Function: spore crust assembly

Product: spore crust anchor protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class III

Gene

Coordinates: 1,249,442 → 1,249,888

Phenotypes of a mutant

• mis-assembly of the spore crust PubMed

The protein

Catalyzed reaction/ biological activity

• required for the localization of most crust proteins PubMed

Paralogous protein(s)

• CotY

Localization

• outermost layer of the spore coat (crust), localization depends on CotE PubMed
• more abundant at the mother cell-proximal pole of the forespore PubMed

Expression and Regulation

Operons

• Genes: cotY-cotZ
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon
  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • GerR: activation, PubMed, in GerR regulon
  • GerE: activation, PubMed, in GerE regulon

  Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed

Biological materials

Mutant

• BKE11740 (ΔcotZ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATCCCTCCTGCCTT, downstream forward: _UP4_TAATCATAAGCTGGAAAAGC
• BKK11740 (ΔcotZ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATCCCTCCTGCCTT, downstream forward: _UP4_TAATCATAAGCTGGAAAAGC

References

Reviews

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Original publications

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