SubtiBank SubtiBank
efeO [2017-06-14 15:56:23]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

efeO [2017-06-14 15:56:23]

lipoprotein, elemental iron uptake system (binding protein), high affinity uptake of ferric iron (Fe(III))
locus
BSU38270
pI
4.71
mw
42.63 kDa
protein length
385 aa Sequence Blast
gene length
1155 bp Sequence Blast
function
elemental iron uptake
product
elemental iron uptake system (binding protein)
essential
no
synonyms
ywbM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.2|Elemental iron transport system]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.5|Elemental iron transport system]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,927,951 → 3,929,108

    The protein

    Catalyzed reaction/ biological activity

  • binds ferric iron (Fe(III)) with strong selectivity [Pubmed|23764491]

    • Protein family

  • UPF0409 family (according to Swiss-Prot)

    • Structure

  • [PDB|3AT7] (from ''Sphingomonas sp.'', 40% identity) [Pubmed|21238429]

    • [SW|Localization]

  • membrane [Pubmed|18763711]
  • extracytosolic lipoprotein [Pubmed|23764491]

    • Expression and Regulation

    Operons

    genes
    [gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]-[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23764491], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9683469], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

    • regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B227 (ywbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1226 NBRP B. subtilis, Japan]

    • References

  • 16672620,9353933,12354229,18763711,23180473,23764491,21238429,27795321