ctaA

heme A synthase

locus: BSU_14870

pI: 9.64

mw: 33.93 kDa

protein length: 306 aa

gene length: 921 bp

function: heme biosynthesis

product: heme A synthase

essential: no

ec: 1.3.-.-

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,558,034 → 1,558,954

The protein

Catalyzed reaction/ biological activity

• heme A synthesis from protoheme IX (heme B)

Protein family

• COX15/ctaA family (single member, according to UniProt)

[SW|Domains]

• contains 8 transmembrane segments

[SW|Cofactors]

• heme B

Structure

• [PDB|6A2J] [pubmed|30397130]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B308BDECB15D68DF0EC2E49341DB5E7F9B3E14D1|ctaA]
  description: [Pubmed|2549007]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11325953], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|2549007,11325953], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|10972837,11325953], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972837,11325953]

Biological materials

Mutant

• BKE14870 (Δ[gene|B308BDECB15D68DF0EC2E49341DB5E7F9B3E14D1|ctaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAATATTCACCTTCTT, downstream forward: _UP4_TAAGGACTCAAGACCAAAGC
• BKK14870 (Δ[gene|B308BDECB15D68DF0EC2E49341DB5E7F9B3E14D1|ctaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAATATTCACCTTCTT, downstream forward: _UP4_TAAGGACTCAAGACCAAAGC

References

Reviews

• 22484221

Original publications

• 15576792,7961419,16321940,11325953,18358840,7968515,15491161,10972837,8665949,10972837,2549007,19174544,26592143,30397130