two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay
function
initiation of sporulation
product
two-component sensor kinase
synonyms
spoIIF, spoIIJ, gsiC, scoD
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue][SW|Categories] containing this gene/protein
[SW|protein modification], [SW|transcription factors and their control], [SW|phosphorelay], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|SigH regulon], [SW|Spo0A regulon], [SW|stringent response]Gene
Coordinates on the chromosome (coding sequence)
1,470,026 -> 1,471,846
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [SW|Spo0F]mainly active in the older, inner regions of a colony (with [protein|search|KinB]) [Pubmed|21097618][SW|Domains]
three tandem [SW|PAS domain]s in the N-terminal region of KinA, the [SW|second PAS] domain is the major N-terminal determinant of KinA dimerization [Pubmed|23504013]the first [SW|PAS domain] is required for NAD( ) binding [Pubmed|23599347]C-terminal histidine phosphotransferase domainModification
autophosphorylation on a His residueEffectors of protein activity
interaction with [protein|search|KipI] or [protein|search|Sda] inhibits autophosphorylation of [protein|search|KinA] [Pubmed|9334321,15023339]autophosphorylation is inhibited by [protein|search|SivA] and [protein|search|BslA] [Pubmed|23335417]activated by interaction with NAD( ) that indicated poor respiratory activity of the cell [Pubmed|23599347]Structure
[PDB|2VLG] (PAS domain)[SW|Localization]
cytoplasm[SW|Interactions]
forms tetramers [Pubmed|21926229][protein|search|KinA]-[protein|search|KipI] [Pubmed|9334321][protein|search|KinA]-[protein|search|Sda] [Pubmed|15023339][SW|Spo0F]-[protein|search|KinA]Expression and Regulation
Operon
''[protein|search|kinA]'' [Pubmed|1569009][SW|Sigma factor]
[protein|search|SigH] [Pubmed|1569009]Regulation
expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|9644251]induced upon addition of decoyinine (positive [SW|stringent response]) [Pubmed|23378509]Regulatory mechanism
[SW|Spo0A]: transcription activation [Pubmed|9644251]Additional information
promoter-up mutations or high artificial expression that render ''[SW|kinA]'' independent from [SW|SigH], allow [SW|sporulation] at high salinity [Pubmed|26712348]References
Reviews
Original Publications
23169620,20511506,19561131,20413551,1846779,11292798,16166384,11734624,9299348,12067336,18174125,10094672,11069677,15023339,2509430,17350039,9334321,9477965,9644251,1569009,20081035,21097618,21926229,22303282,22670053,23335417,23378509,23504013,23599347,25598361,26055117,26165942,26657919,26712348 
