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yfhF [2019-01-02 18:32:10]
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yfhF [2019-01-02 18:32:10]

general stress protein, similar to nucleoside-diphosphate sugar epimerase
locus
BSU08510
pI
9.93
mw
33.79 kDa
protein length
303 aa Sequence Blast
gene length
909 bp Sequence Blast
function
survival of ethonol stress and at low temperatures
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

    • Gene

    Coordinates
    924,633 → 925,544

    Phenotypes of a mutant

  • poorly transformable [pubmed|28189581]

    • The protein

    Protein family

  • SDR39U1 subfamily (according to Swiss-Prot)

    • Structure

  • [PDB|3OH8] (from Corynebacterium glutamicum, 32% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|B2924DAA879FBE56EB8DF9A7C60222AA1EE5526C|yfhF]-[gene|EE8065E719D0359B5892C8DCBCEC0B246ABE7D5B|yfhE]-[gene|29A9F7EEC3EFD8CA913877C41C1F00BCE9FF384D|yfhD]
    description
    [Pubmed|27965289]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|15699190]

    • additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|29A9F7EEC3EFD8CA913877C41C1F00BCE9FF384D|YfhD]' [PubMed|20525796]
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    Biological materials

    Mutant

  • MGNA-C315 (yfhF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2313 NBRP B. subtilis, Japan]
  • BKE08510 (Δ[gene|B2924DAA879FBE56EB8DF9A7C60222AA1EE5526C|yfhF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACATCTCTCCTCATC, downstream forward: _UP4_TAAAAACAGCAGGAAGCGTC
  • BKK08510 (Δ[gene|B2924DAA879FBE56EB8DF9A7C60222AA1EE5526C|yfhF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACATCTCTCCTCATC, downstream forward: _UP4_TAAAAACAGCAGGAAGCGTC

    • References

  • 15805528,28189581