yfhF

general stress protein, similar to nucleoside-diphosphate sugar epimerase

locus: BSU_08510

pI: 9.93

mw: 33.79 kDa

protein length: 303 aa

gene length: 912 bp

function: survival of ethonol stress and at low temperatures

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 924,633 → 925,544

Phenotypes of a mutant

• poorly transformable [pubmed|28189581]

The protein

Protein family

• [SW|NAD(P)-dependent epimerase/dehydratase family] (according to UniProt)

Structure

• [PDB|3OH8] (from Corynebacterium glutamicum, 32% identity)

Expression and Regulation

Operons

• genes: [gene|B2924DAA879FBE56EB8DF9A7C60222AA1EE5526C|yfhF]-[gene|EE8065E719D0359B5892C8DCBCEC0B246ABE7D5B|yfhE]-[gene|29A9F7EEC3EFD8CA913877C41C1F00BCE9FF384D|yfhD]
  description: [Pubmed|27965289]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|15699190]

  additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|29A9F7EEC3EFD8CA913877C41C1F00BCE9FF384D|YfhD]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-C315 (yfhF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2313 NBRP B. subtilis, Japan]
• BKE08510 (Δ[gene|B2924DAA879FBE56EB8DF9A7C60222AA1EE5526C|yfhF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACATCTCTCCTCATC, downstream forward: _UP4_TAAAAACAGCAGGAAGCGTC
• BKK08510 (Δ[gene|B2924DAA879FBE56EB8DF9A7C60222AA1EE5526C|yfhF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACATCTCTCCTCATC, downstream forward: _UP4_TAAAAACAGCAGGAAGCGTC

References

• 15805528,28189581