pheT [2019-08-08 10:16:26]

phenylalanyl-tRNA synthetase (beta subunit)

Locus: BSU_28630

Isoelectric point: 4.86

Molecular weight: 87.76 kDa

Protein length: 804 aa

Gene length: 2415 bp

Function: translation

Product: phenylalanyl-tRNA synthetase (beta subunit)

Essential: yes

E.C.: 6.1.1.20

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Aminoacyl-tRNA synthetases
• Groups of genes → Essential genes

Gene

Coordinates: 2,927,008 → 2,929,422

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)

Protein family

• phenylalanyl-tRNA synthetase beta subunit family (single member, according to UniProt)

Structure

• 1PYS (the PheS-PheT complex from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: pheS-pheT
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • T-box: transcriptional antitermination, via tRNA controlled RNA switch, in T-box

  Regulation

  • induced by phenylalanine limitation (T-box) PubMed
  • the T-box RNA is degraded by RNase Y PubMed

References

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