adhR [2018-02-23 17:49:48]

transcriptional activator (MerR family) of adhA-yraA, responsive to formaldehyde and methylglyoxal

locus: BSU27000

pI: 9.64

mw: 16.30 kDa

protein length: 140 aa

gene length: 420 bp

function: regulation of the protective response to formaldehyde and methylglyoxal

product: transcriptional activator (MerR family)

essential: no

synonyms: yraB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,755,382 → 2,755,804

The protein

Protein family

• [SW|MerR family]

Modification

• activity probably redox-controlled via thiol-(S)-alkylation at Cys-52 by aldehydes [Pubmed|19170879]

[SW|Localization]

• cytoplasmic

Expression and Regulation

Operons

• genes: [gene|B24BA6ABCAF7325EC5F8B2655CA182FFC23395FD|adhR]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A238 (yraB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/238 NBRP B. subtilis, Japan]
• BKE27000 (Δ[gene|B24BA6ABCAF7325EC5F8B2655CA182FFC23395FD|adhR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCACCTCTCTAT, downstream forward: _UP4_TAGCATTCTTTGCGTGTGAA
• BKK27000 (Δ[gene|B24BA6ABCAF7325EC5F8B2655CA182FFC23395FD|adhR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCACCTCTCTAT, downstream forward: _UP4_TAGCATTCTTTGCGTGTGAA

Labs working on this gene/protein

• [SW|Haike Antelmann],University of Greifswald, Germany

References

• 21722790