tcyN

cystine ABC transporter (ATP-binding protein)

Locus: BSU_29340

Isoelectric point: 8.84

Molecular weight: 29.41 kDa

Protein length: 259 aa

Gene length: 780 bp

Function: cystine uptake

Product: cystine ABC transporter (ATP-binding protein)

Essential: no

Synonyms: hisP,ytmN

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of amino acids
• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,004,346 → 3,005,125

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Paralogous protein(s)

• TcyC, YxeO, PstBB, YvrO, GlnQ, ArtR, PstBA

Domains

• ABC transporter domain (aa 2-239) (according to UniProt)

Structure

• 2Q0H (complex with ADP-Mg2+, Geobacillus stearothermophilus, 54% identity )

Localization

• attached to the membrane via TcyL-TcyM PubMed

Expression and Regulation

Operons

• Genes: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • AscR: activation, PubMed, in AscR regulon

  Regulation

  • induced in the presence of methionine and taurine PubMed

Biological materials

Mutant

• BKE29340 (ΔtcyN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTATGGATATTTTTAATCT, downstream forward: _UP4_GAACACATAAAGGAGCCGGT
• BKK29340 (ΔtcyN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTATGGATATTTTTAATCT, downstream forward: _UP4_GAACACATAAAGGAGCCGGT

References

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