gyrA [2020-01-26 12:29:03]

DNA gyrase (subunit A)

locus: BSU_00070

pI: 5.22

mw: 91.91 kDa

protein length: 821 aa

gene length: 2466 bp

function: DNA supercoiling, control of [category|SW 3.1.1|replication] initiation

product: DNA gyrase (subunit A)

essential: yes

ec: 5.99.1.3

synonyms: nalA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 6,994 → 9,459

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)
• decreases [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] association with the oriC and inhibits [category|SW 3.1.1|replication] initiation [pubmed|29396913]

Protein family

• Topoisomerase IIA [protein|B207C7328177924B16F1C606F667BEADF12CA069|GyrA]/[protein|9075DD0CC0B8554F0379732453665F43C70EFC26|ParC] subunit family (together with [protein|9075DD0CC0B8554F0379732453665F43C70EFC26|ParC]) (according to UniProt)

Paralogous protein(s)

• [protein|9075DD0CC0B8554F0379732453665F43C70EFC26|ParC]

Structure

• [PDB|4DDQ] (DNA gyrase A-subunit lacking the C-terminal DNA-wrapping domains) [Pubmed|23602808]

[SW|Localization]

• Nucleoid (Homogeneous) [Pubmed|16479537]
• nucleoid-associated in actively growing cells [Pubmed|9539793]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

labs

• [SW|Dagmar Klostermeier], University of Münster, Germany, [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]

References

Reviews

• 16936698

Original publications

• 16479537,17981983,9539793,2987848,19666507,17320901,23475963,23602808,20870749,24563461,27557712,27793284,12767818,6282812,29396913,8940171,7590259,10944214,6248518,6243420,225110,17334374,30457554,30517674,31953321