phy [2019-09-17 10:49:13]

phytase

locus: BSU_19800

pI: 4.94

mw: 41.79 kDa

protein length: 382 aa

gene length: 1149 bp

function: utilization of inositol hexakisphosphate (phytate)

product: 3-phytase

essential: no

ec: 3.1.3.8

synonyms: yodV,yzxA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,150,108 → 2,151,256

The protein

Catalyzed reaction/ biological activity

• 1D-myo-inositol hexakisphosphate + H₂O --> 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate (according to UniProt)

Structure

• [PDB|3AMR]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|B1F5776C9882CDA4D97F2A2FBB01FAB34D21022B|phy]
  description: [Pubmed|7592393]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  additional information

  • the gene is not expressed in 'B. subtilis' due to the absene of a functional promoter [PubMed|16980498]

Biological materials

Mutant

• MGNA-B509 (yodV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1508 NBRP B. subtilis, Japan]
• BKE19800 (Δ[gene|B1F5776C9882CDA4D97F2A2FBB01FAB34D21022B|phy]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTACCCTCCTCTTT, downstream forward: _UP4_TAGAATAGAAAGCAGCTTGT
• BKK19800 (Δ[gene|B1F5776C9882CDA4D97F2A2FBB01FAB34D21022B|phy]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTACCCTCCTCTTT, downstream forward: _UP4_TAGAATAGAAAGCAGCTTGT

References

• 18957862,24407511,10655618,7592393,16980498,20601512,23178368,20817675,26106383