tcyM

cystine [SW|ABC transporter] (permease)

locus: BSU_29350

pI: 8.77

mw: 25.45 kDa

protein length: 235 aa

gene length: 708 bp

function: cystine uptake

product: cystine [SW|ABC transporter] (permease)

essential: no

synonyms: ytmM

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,005,122 → 3,005,829

The protein

Protein family

• [SW|Binding-protein-dependent transport system permease family] (according to UniProt)

[SW|Domains]

• [SW|ABC transmembrane type-1 domain] (aa 15-220) (according to UniProt)

Structure

• [PDB|4YMS] (amino acid transporter from Caldanaerobacter subterraneus, 31% identity) [pubmed|25848002]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
  description: [Pubmed|15668000]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15272571], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16109943], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|741156D495BE3857683C8A0390764EAD83845ABC|AscR]: activation, [Pubmed|16109943], in [regulon|741156D495BE3857683C8A0390764EAD83845ABC|AscR regulon]

  regulation

  • induced in the presence of methionine and taurine [Pubmed|11390694]

Biological materials

Mutant

• MGNA-A173 (ytmM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/173 NBRP B. subtilis, Japan]
• 1A948 ( ''tcyM''::''kan''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A948&Search=1A948 BGSC]
• BKE29350 (Δ[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTTCTCCCCCCTCATC, downstream forward: _UP4_GCCAGATTTCAAGGAGGCAC
• BKK29350 (Δ[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTTCTCCCCCCTCATC, downstream forward: _UP4_GCCAGATTTCAAGGAGGCAC

References

• 15262924,10092453,16109943,12193636,15668000,15272571,23944997,25848002