yfjN

putative tRNA-dihydrouridine synthase 2

locus: BSU_08030

pI: 5.38

mw: 36.83 kDa

protein length: 325 aa

gene length: 978 bp

function: tRNA modification

product: putative tRNA-dihydrouridine synthase 2

essential: no

synonyms: dusC,dus2

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 876,426 → 877,403

The protein

Catalyzed reaction/ biological activity

• 5,6-dihydrouridine in tRNA + NAD⁺ → uridine in tRNA + H⁺ + NADH (according to UniProt)
• 5,6-dihydrouridine in tRNA + NADP⁺ → uridine in tRNA + H⁺ + NADPH (according to UniProt)

Protein family

• Dus family (with [protein|CE45D56BC413555AF4E7914B5307AD967A358452|YacF], according to UniProt)

Paralogous protein(s)

• [protein|CE45D56BC413555AF4E7914B5307AD967A358452|YacF]

[SW|Cofactors]

• FMN or FAD [Pubmed|21635694]

Structure

• [PDB|3B0P] (from Thermus thermophilus, 24% identity, from aa 12 ... 263) [pubmed|22123979]

Expression and Regulation

Operons

• genes: [gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]
  description: [Pubmed|29794222]

  regulation

  • the leader mRNA is processed by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

Mutant

• MGNA-C277 (yfjN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2275 NBRP B. subtilis, Japan]
• BKE08030 (Δ[gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCA
• BKK08030 (Δ[gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCA

References

• 25902496,22123979,29794222