yfjN

putative tRNA-dihydrouridine synthase 2

Locus: BSU_08030

Isoelectric point: 5.38

Molecular weight: 36.83 kDa

Protein length: 325 aa

Gene length: 978 bp

Function: tRNA modification

Product: putative tRNA-dihydrouridine synthase 2

Essential: no

Synonyms: dusC,dus2

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Gene

Coordinates: 876,426 → 877,403

The protein

Catalyzed reaction/ biological activity

• 5,6-dihydrouridine in tRNA + NAD⁺ → uridine in tRNA + H⁺ + NADH (according to UniProt)
• 5,6-dihydrouridine in tRNA + NADP⁺ → uridine in tRNA + H⁺ + NADPH (according to UniProt)

Protein family

• Dus family (with YacF, according to UniProt)

Paralogous protein(s)

• YacF

Cofactors

• FMN or FAD PubMed

Structure

• 3B0P (from Thermus thermophilus, 24% identity, from aa 12 ... 263) PubMed

Expression and Regulation

Operons

• Genes: yfjN
  Description: PubMed

  Regulation

  • the leader mRNA is processed by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

Biological materials

Mutant

• MGNA-C277 (yfjN::erm), available at the NBRP B. subtilis, Japan
• BKE08030 (ΔyfjN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCA
• BKK08030 (ΔyfjN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCA

References

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