ywpE

similar to sortase, but lacking the membrane anchor

locus: BSU_36340

pI: 6.34

mw: 11.00 kDa

protein length: 102 aa

gene length: 309 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 3,741,732 → 3,742,040

The protein

Protein family

• bacterial sortase family (with [protein|A24243129860F1B043EBE1358FAEA57BED111BBC|YhcS], according to UniProt)

Structure

• [PDB|5HU4] (sortase from Listeria monocytogenes, 49% identity) [pubmed|26826492]

Expression and Regulation

Operons

• genes: [gene|B17670494CC6F7C1138F4B4ECC0FEE8FC15609A2|ywpE]
  description: [Pubmed|9353933]

  regulation

  • strongly repressed during oligotrophic growth [pubmed|30792386]

Biological materials

Mutant

• MGNA-A588 (ywpE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/588 NBRP B. subtilis, Japan]
• BKE36340 (Δ[gene|B17670494CC6F7C1138F4B4ECC0FEE8FC15609A2|ywpE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTGCCGCCCCTATCA, downstream forward: _UP4_TAAATATGCAAGAGTGAAAG
• BKK36340 (Δ[gene|B17670494CC6F7C1138F4B4ECC0FEE8FC15609A2|ywpE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTGCCGCCCCTATCA, downstream forward: _UP4_TAAATATGCAAGAGTGAAAG

References

Reviews

• 22026821

Research papers

• 26826492,30792386