ureB

urease (beta subunit)

Locus: BSU_36650

Isoelectric point: 9.75

Molecular weight: 13.50 kDa

Protein length: 124 aa

Gene length: 375 bp

Function: utilization of urea as alternative nitrogen source

Product: urease (beta subunit)

Essential: no

E.C.: 3.5.1.5

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of urea

Gene

Coordinates: 3,768,420 → 3,768,794

The protein

Catalyzed reaction/ biological activity

• 2 H⁺ + H₂O + urea --> CO₂ + 2 NH₄⁺ (according to UniProt)

Protein family

• urease beta subunit family (single member, according to UniProt)

Structure

• 3UBP (from B. pasteurii, 48% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ywzF-ywzE-ureA-ureB-ureC
  Description: PubMed

• Genes: ureA-ureB-ureC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigH: sigma factor, PubMed, in SigH regulon

  Regulatory mechanism

  • GlnR: repression, PubMed, in GlnR regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • PucR: activation, PubMed, in PucR regulon
  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • induced by nitrogen limitation (GlnR, TnrA) PubMed

Biological materials

Mutant

• BKE36650 (ΔureB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGCAATTTGAAATGCTCCCG, downstream forward: _UP4_GGCTGGATGGAGGGTGTAAT
• BKK36650 (ΔureB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGCAATTTGAAATGCTCCCG, downstream forward: _UP4_GGCTGGATGGAGGGTGTAAT

References

Reviews

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Original publications

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