glpF

glycerol facilitator

Locus: BSU_09280

Isoelectric point: 9.51

Molecular weight: 28.59 kDa

Protein length: 274 aa

Gene length: 825 bp

Function: glycerol uptake

Product: glycerol facilitator

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Carbohydrate transporter
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glycerol/ glycerol-3-phosphate
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,002,501 → 1,003,325

The protein

Protein family

• MIP/aquaporin (TC 1.A.8) family (single member, according to UniProt)

Structure

• 1FX8 (the protein from E. coli, 37% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: glpF-glpK
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • GlpP: antitermination, /antitermination via GlpP-dependent RNA switch, in GlpP regulon
  • AbrB: activation, PubMed, in AbrB regulon
  • ResD: repression, PubMed, in ResD regulon

  Regulation

  • induction by glycerol (GlpP) PubMed
  • repressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• GP99 (cat) (available in Jörg Stülke's lab)
• BKE09280 (ΔglpF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGCACATTCCTCCTAAA, downstream forward: _UP4_ATTTAATCAAAGGGGAGACA
• BKK09280 (ΔglpF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGCACATTCCTCCTAAA, downstream forward: _UP4_ATTTAATCAAAGGGGAGACA

References

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