yabO [2019-03-19 19:15:16]

similar to heat shock protein Hsp15

locus: BSU00590

pI: 10.14

mw: 9.58 kDa

protein length: 86 aa

gene length: 261 bp

function: recycling of stalled ribosomes

product: Hsp15

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.13|Translation/ other/ based on similarity]

Gene

Coordinates: 67,877 → 68,137

The protein

Catalyzed reaction/ biological activity

• translocates the tRNA moiety from the A site to the P site of stalled 50S ribosomal subunits [pubmed|19013177]

Structure

• [PDB|3BBU] (from E. coli, 36% identity) [pubmed|19013177]

Expression and Regulation

Operons

• genes: [gene|D6B1837075ECBEE1FEAC704DDB6795336092897F|yabM]-[gene|52D48F22CB53421EE30970D34B4861E2F8A5893D|yabN]-[gene|B0CB19A3E3E84E12BF42BB050394C8A059A89359|yabO]-[gene|035B5486A3887FC71ACC0A8FB43ED77DC44E12EA|yabP]-[gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|yabQ]-[gene|FE0E60B875E72AEF5174A89C7AE5556FF59B19D5|divIC]-[gene|8EDB1BCEEE64FDAF1C9054300751FCB420658865|yabR]
  description: [Pubmed|11283287]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,11283287], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|26577401], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|12207695], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulation

  • expressed during sporulation ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|11283287]

Biological materials

Mutant

• MGNA-B916 (yabO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1915 NBRP B. subtilis, Japan]
• BKE00590 (Δ[gene|B0CB19A3E3E84E12BF42BB050394C8A059A89359|yabO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAATTTATCTAATCTCA, downstream forward: _UP4_TAGGCTTGTTCTAAAAAAAC
• BKK00590 (Δ[gene|B0CB19A3E3E84E12BF42BB050394C8A059A89359|yabO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAATTTATCTAATCTCA, downstream forward: _UP4_TAGGCTTGTTCTAAAAAAAC

References

• 11283287,10675343,10675344,8349564,9867837,19013177,26577401