katA [2019-07-25 10:21:28]

main vegetative catalase 1

Locus: BSU_08820

Isoelectric point: 6.15

Molecular weight: 54.57 kDa

Protein length: 483 aa

Gene length: 1452 bp

Function: detoxification (degradation) of hydrogen peroxide

Product: vegetative catalase

Essential: no

E.C.: 1.11.1.6

Synonyms: kat-19

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 959,535 → 960,986

Phenotypes of a mutant

• increased sensitivity to oxidative stress PubMed
• increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage PubMed

The protein

Catalyzed reaction/ biological activity

• 2 H₂O₂ = O₂ + 2 H₂O (according to Swiss-Prot)

Protein family

• catalase family (according to UniProt)

Paralogous protein(s)

• KatX, KatE

Modification

• phosphorylated on Arg-366 PubMed

Cofactors

• contains an iron-sulfur cluster, heme

Structure

• 1SI8 (enzyme from Enterococcus faecalis, 68% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: katA
  Description: PubMed

  Regulatory mechanism

  • PerR: repression, PubMed, in PerR regulon

  Regulation

  • induced in the presence of hydrogen peroxide and paraquat (PerR) PubMed

Biological materials

Mutant

• GP1726 katA::xeo trpC2 available at Jörg Stülkes lab
• GP1725 katA::cat trpC2 available at Jörg Stülkes lab
• BKE08820 (ΔkatA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTATCACCTCTTGGAA, downstream forward: _UP4_TAATGGAGAAATGCAAAAAC
• BKK08820 (ΔkatA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTATCACCTCTTGGAA, downstream forward: _UP4_TAATGGAGAAATGCAAAAAC

References

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