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katA [2019-07-25 10:21:28]
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katA [2019-07-25 10:21:28]

main vegetative catalase 1
Locus
BSU_08820
Isoelectric point
6.15
Molecular weight
54.57 kDa
Protein length
483 aa Sequence Blast
Gene length
1452 bp Sequence Blast
Function
detoxification (degradation) of hydrogen peroxide
Product
vegetative catalase
Essential
no
E.C.
1.11.1.6
Synonyms
kat-19

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
959,535 → 960,986

Phenotypes of a mutant

  • increased sensitivity to oxidative stress PubMed
  • increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • 2 H2O2 = O2 + 2 H2O (according to Swiss-Prot)
  • Protein family

  • catalase family (according to UniProt)
  • Paralogous protein(s)

    Modification

  • phosphorylated on Arg-366 PubMed
  • Cofactors

  • contains an iron-sulfur cluster, heme
  • Structure

  • 1SI8 (enzyme from Enterococcus faecalis, 68% identity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • PerR: repression, PubMed, in PerR regulon
  • Regulation

  • induced in the presence of hydrogen peroxide and paraquat (PerR) PubMed
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    Biological materials

    Mutant

  • GP1726 katA::xeo trpC2 available at Jörg Stülkes lab
  • GP1725 katA::cat trpC2 available at Jörg Stülkes lab
  • BKE08820 (ΔkatA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTATCACCTCTTGGAA, downstream forward: _UP4_TAATGGAGAAATGCAAAAAC
  • BKK08820 (ΔkatA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTATCACCTCTTGGAA, downstream forward: _UP4_TAATGGAGAAATGCAAAAAC
  • References

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