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cwlD [2019-06-17 12:07:16]
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cwlD [2019-06-17 12:07:16]

N-acetylmuramoyl-L-alanine amidase, spore cortex peptidoglycan synthesis
Locus
BSU01530
Isoelectric point
9.79
Molecular weight
26.86 kDa
Protein length
237 aa Sequence Blast
Gene length
714 bp Sequence Blast
Function
spore cortex peptidoglycan synthesis
Product
N-acetylmuramoyl-L-alanine amidase
Essential
no
E.C.
3.5.1.28
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
156,612 → 157,325

The protein

Catalyzed reaction/ biological activity

  • formation of muramic acid delta-lacton in spore cortex peptidoglycan PubMed
  • Protein family

  • N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
  • Domains

  • contains an amidase_3 domain (like CwlC, LytC, YqiI, YrvJ)
  • Structure

  • 4RN7 (from Clostridium difficile, 30% identity, aa 43 - 234)
  • Localization

  • secreted (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigG: sigma factor, PubMed, in SigG regulon
  • Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon
  • Regulation

  • expressed during sporulation (SigG) PubMed
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    Genes
    Description

    Regulation

  • expressed during sporulation (SigG, SigE) PubMed
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    Biological materials

    Mutant

  • BKE01530 (ΔcwlD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG
  • BKK01530 (ΔcwlD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG
  • References

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