SubtiBank SubtiBank
cwlD
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cwlD

N-acetylmuramoyl-L-alanine amidase, spore cortex peptidoglycan synthesis
Locus
BSU_01530
Isoelectric point
9.79
Molecular weight
26.86 kDa
Protein length
237 aa Sequence Blast
Gene length
714 bp Sequence Blast
Function
spore cortex peptidoglycan synthesis
Product
N-acetylmuramoyl-L-alanine amidase
Essential
no
E.C.
3.5.1.28
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
156,612 → 157,325

The protein

Catalyzed reaction/ biological activity

  • formation of muramic acid delta-lacton in spore cortex peptidoglycan PubMed
  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
  • Protein family

    Domains

  • contains an amidase_3 domain (like CwlC, LytC, YqiI, YrvJ)
  • MurNAc-LAA domain (aa 43-226) (according to UniProt)
  • Structure

  • 4RN7 (from Clostridium difficile, 30% identity, aa 43 - 234)
  • Localization

  • secreted (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigG: sigma factor, PubMed, in SigG regulon
  • Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon
  • Regulation

  • expressed during sporulation (SigG) PubMed
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    Genes
    Description

    Regulation

  • expressed during sporulation (SigG, SigE) PubMed
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    Biological materials

    Mutant

  • BKE01530 (ΔcwlD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG
  • BKK01530 (ΔcwlD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG
  • References

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