yqkF [2018-02-07T17:14:01.000Z]

NADPH-dependent 4-Hydroxy-2,3-trans-nonenal reductase

locus: BSU23620

pI: 5.13

mw: 34.56 kDa

protein length: 306 aa

gene length: 918 bp

function: unknown

product: NADPH-dependent 4-Hydroxy-2,3-trans-nonenal reductase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,459,326 → 2,460,246

The protein

Protein family

• aldo/keto reductase 2 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|F1AFFACD36C75702B0BB8F0B4241A9688FB6253C|IolS] (31,4%)

[SW|Cofactors]

• NADPH [Pubmed|16242712]

Structure

• [PDB|1YNP] (from ''B. halodurans'', 64% identity) [Pubmed|16242712]

Expression and Regulation

Operons

• genes: [gene|B0897643562CDB25547223CCB9CFD0F9E78710E2|yqkF]
  description: (according to [http://dbtbs.hgc.jp/COG/prom/yqkF.html DBTBS]) null

• genes: [gene|703D69B351D8DBF9FE84113955DFF509819D0359|mciZ]-[gene|B0897643562CDB25547223CCB9CFD0F9E78710E2|yqkF]
  description: [pubmed|22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [pubmed|18284588], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|18284588]
  • strongly repressed in the presence of salt (1.2 M NaCl) [pubmed|32419322]

Biological materials

Mutant

• BKE23620 (Δ[gene|B0897643562CDB25547223CCB9CFD0F9E78710E2|yqkF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGCACACTCCTTTTTT, downstream forward: _UP4_TAGCAGAAAAACCAGCACCT
• BKK23620 (Δ[gene|B0897643562CDB25547223CCB9CFD0F9E78710E2|yqkF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGCACACTCCTTTTTT, downstream forward: _UP4_TAGCAGAAAAACCAGCACCT