yjcD [2019-09-05 09:40:00]

similar to ATP-dependent DNA helicase

locus: BSU_11820

pI: 8.02

mw: 86.79 kDa

protein length: 717 aa

gene length: 2154 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.6|DNA repair/ recombination/ based on similarity]

Gene

Coordinates: 1,253,713 → 1,255,866

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• [SW|helicase family] (according to UniProt)

Paralogous protein(s)

• [protein|C73E52D214E24F21696973B19B0A44CE785D6FBA|PcrA]

Structure

• [PDB|2PJR] ([protein|C73E52D214E24F21696973B19B0A44CE785D6FBA|PcrA] product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'', 36% identity, 55% similarity)
• [PDB|1PJR] ([protein|C73E52D214E24F21696973B19B0A44CE785D6FBA|PcrA] substrate complex, complex with DNA, ''Geobacillus stearothermophilus'', 36% identity, 55% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B0789A727D9E3578FF1CB6162F365F45FADB0B3E|yjcD]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A159 (yjcD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/159 NBRP B. subtilis, Japan]
• BKE11820 (Δ[gene|B0789A727D9E3578FF1CB6162F365F45FADB0B3E|yjcD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACGGGCTTTCCGCAAT, downstream forward: _UP4_TAACAGCCCGGCAGATTACT
• BKK11820 (Δ[gene|B0789A727D9E3578FF1CB6162F365F45FADB0B3E|yjcD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACGGGCTTTCCGCAAT, downstream forward: _UP4_TAACAGCCCGGCAGATTACT

References

• 10199404