cheB [2017-07-18 12:15:44]

MCP-glutamate methylesterase / two-component response regulator-like

Locus: BSU16420

Isoelectric point: 8.12

Molecular weight: 38.49 kDa

Protein length: 357 aa

Gene length: 1071 bp

Function: motility and chemotaxis

Product: MCP-glutamate methylesterase / two-component response regulator-like

Essential: no

E.C.: 3.1.1.61

Synonyms: cheL

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Soluble signalling proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 1,711,736 → 1,712,809

The protein

Catalyzed reaction/ biological activity

• Protein L-glutamate O(5)-methyl ester + H₂O = protein L-glutamate + methanol (according to Swiss-Prot)

Modification

• phosphorylated on a Asp residue by CheA PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • CodY: repression, in CodY regulon
  • DegU: regulation, in DegU regulon
  • SwrA: activation, in SwrA regulon

  Regulation

  • see fla-che operon

• Genes: flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • SwrA: activation, in SwrA regulon
  • CodY: repression, in CodY regulon
  • DegU: repression, in DegU regulon

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

Biological materials

Mutant

• DS7306 (marker-less in NCIB3610) PubMed

Labs working on this gene/protein

• Daniel Kearns

References

Reviews

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Original publications

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