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cdaA [Wed Aug 10 2016 16:30:49 GMT+0200 (CEST)]
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cdaA [Wed Aug 10 2016 16:30:49 GMT+0200 (CEST)]

diadenylate cyclase, synthesis of c-di-AMP in vegetative cells
locus
BSU01750
pI
8.00
mw
30.00 kDa
function
synthesis of c-di-AMP
product
diadenylate cyclase
essential
no
ec
2.7.7.85
synonyms
ybbP, ybbQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|membrane proteins], [SW|metabolism of signalling nucleotides]

    • Gene

    Coordinates on the chromosome (coding sequence)
    196,213 -> 197,034

    Phenotypes of a mutant

  • inactivation of ''[protein|search|cdaA]'' results in severe beta-lactam sensitivity [Pubmed|22211522]

    • The protein

    Catalyzed reaction/ biological activity

  • synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352]

    • Paralogous protein(s)

  • [protein|search|CdaS]

    • [SW|Domains]

  • contains a [SW|DAC domain] involved in the synthesis of c-di-AMP [Pubmed|21566650]

    • Effectors of protein activity

  • the interaction with [protein|search|CdaR] stimulates the diadenylate cyclase activity of [protein|search|CdaA] [Pubmed|23192352]

    • Structure

  • [PDB|4RV7] (the [SW|DAC domain] and C-terminal domain of CdaA from ''Listeria monocytogenes'' (aa 101 - 273), 65% identity) [Pubmed|25605729]

    • [SW|Localization]

  • cell membrane [Pubmed|26240071]

    • [SW|Interactions]

  • [protein|search|CdaA]-[protein|search|CdaR], to control [protein|search|CdaA] activity [Pubmed|23192352]
  • [protein|search|CdaA]-[protein|search|GlmM] [Pubmed|26240071]

    • Expression and Regulation

    Operon

  • ''[protein|search|cdaA]-[protein|search|cdaR]-[protein|search|glmM]-[protein|search|glmS]'' [Pubmed|23192352]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|22211522]

    • Additional information

  • the '[protein|search|cdaA]-[protein|search|cdaR]' part of the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

    • Biological materials

    Mutant

  • GP94 (D''[protein|search|cdaA]''::''spc''), available in [SW|Jörg Stülke]'s lab
  • GP997 (''[protein|search|cdaA]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • GP985 (''[protein|search|cdaA]''-''[protein|search|cdaR]''::''cat''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • expression of native ''cdaA'' in ''B. subtilis'': pGP1960 (in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • expression of ''cdaA''-Strep in ''B. subtilis'' suitable for [SW|SPINE]: pGP1986 (in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
  • IPTG inducible expression of ''cdaA''-Strep in ''E. coli'': pGP2564 (in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • GP1339 (cat) based on [protein|search|pAC6], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab. Respective plasmid: pGP1990.

    • FLAG-tag construct

  • GP1381 ''cdaA-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 18714086,25869574,23812326

    • Original publications

  • 12884008,21566650,23192352,22211522,25605729,25616256,26240071,26527648