xylR

transcriptional repressor of the [gene|24829427B4518FE67038F194B54D4AAFAC64EF19|xylA]-[gene|search|xylB ]and [gene|F92925E2FDCFE6DDA3E87C7B541FE6A6CE5AABB8|xynP]-[gene|search|xynB ]operons

locus: BSU_17590

pI: 5.82

mw: 38.29 kDa

protein length: 350 aa

gene length: 1053 bp

function: regulation of xylan and xylose utilization

product: transcriptional repressor

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.5|Utilization of xylan/ xylose]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 1,890,512 → 1,891,564

The protein

Catalyzed reaction/ biological activity

• transcription repression of the ''[gene|24829427B4518FE67038F194B54D4AAFAC64EF19|xylA]-[gene|F417A5B46CC7FC69DC8606A46D1AD5E41FA35B13|xylB]'' and the ''[gene|F92925E2FDCFE6DDA3E87C7B541FE6A6CE5AABB8|xynP]-[gene|19CD9BF216953613CD2E0DFB99D4C6E92AAFAE5D|xynB]'' operons

Protein family

• ROK (NagC/XylR) family (with [protein|EB6DE119E6FB18413DB2C2B62B114FB787164F74|GmuE] and [protein|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|GlcK], according to UniProt)

Paralogous protein(s)

• [protein|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|GlcK]

Effectors of protein activity

• inducer: xylose

Structure

• [PDB|5F7R] (Rok from Listeria monocytogenes, 28% identity) [pubmed|27819654]

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000089 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|AF4395D134485290F4AA307B48494FED39E52CD7|xylR]
  description: [pubmed|22383849]

Biological materials

Mutant

• GP270 (erm), GP1302 (aphA3), available in the [SW|Stülke] lab
• BKE17590 (Δ[gene|AF4395D134485290F4AA307B48494FED39E52CD7|xylR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATCCACTCCATTTG, downstream forward: _UP4_TAATTTTTTATGGAATGGAC
• BKK17590 (Δ[gene|AF4395D134485290F4AA307B48494FED39E52CD7|xylR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATCCACTCCATTTG, downstream forward: _UP4_TAATTTTTTATGGAATGGAC

labs

• [SW|Wolfgang Hillen], Erlangen University, Germany

References

• 7559331,7966270,25777134,7952186,8319885,2544559,1588910,27819654