yhcV [2017-8-11 16:0:3]

forespore-specific sporulation protein

locus: BSU09230

pI: 5.01

mw: 14.77 kDa

protein length: 140 aa

gene length: 420 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 997,175 → 997,597

The protein

Paralogous protein(s)

• [protein|8529D83560E934709B20CCF0B72251A74EC272BB|YlbB]

[SW|Domains]

• 2 [SW|CBS domain]s (8-64, 72-127)

Structure

• [PDB|4FRY] (protein from ''Burkholderia ambifaria'', 30% identity) [Pubmed|23382856]

Additional information

• The gene is annotated in KEGG as an ortholog of IMP dehydrogenase EC 1.1.1.205. No EC annotation is available in Swiss-Prot/MetaSwiss-Prot/MetaCyc.literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

Expression and Regulation

Operons

• genes: [gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]
  description: [Pubmed|12480901]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190,12480901,16497325], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|15699190,12480901,16497325]

Biological materials

Mutant

• MGNA-B474 (yhcV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1473 NBRP B. subtilis, Japan]

Expression vector

• pGP2930 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

• 15699190,12480901,16497325,23382856