yhcV

forespore-specific sporulation protein

Locus: BSU_09230

Isoelectric point: 5.01

Molecular weight: 14.77 kDa

Protein length: 140 aa

Gene length: 423 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 997,175 → 997,597

The protein

Paralogous protein(s)

• YlbB:

Domains

• 2 CBS domains (aa 8-64, aa 72-127) (according to UniProt)

Structure

• 4FRY (protein from Burkholderia ambifaria, 30% identity) PubMed

Additional information

• The gene is annotated in KEGG as an ortholog of IMP dehydrogenase EC 1.1.1.205. No EC annotation is available in Swiss-Prot/MetaSwiss-Prot/MetaCyc.literature/experimental evidence supporting the annotation is available. PubMed

Expression and Regulation

Operons

• Genes: yhcV
  Description: PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

  Regulation

  • expressed during sporulation in the forespore (SigG) PubMed

Additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores PubMed

Biological materials

Mutant

• MGNA-B474 (yhcV::erm), available at the NBRP B. subtilis, Japan
• BKE09230 (ΔyhcV::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA, downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG
• BKK09230 (ΔyhcV::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA, downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG

Expression vectors

• pGP2930 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

References

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