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aspB
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aspB

aspartate transaminase
Locus
BSU_22370
Isoelectric point
5.30
Molecular weight
42.93 kDa
Protein length
393 aa Sequence Blast
Gene length
1182 bp Sequence Blast
Function
biosynthesis of aspartate
Product
aspartate transaminase
Essential
no
E.C.
2.6.1.1
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,347,660 2,348,841

Phenotypes of a mutant

  • auxotrophic for aspartate and asparagine PubMed
  • lyses on DSM dporulation medium due to the inhibition of GltT-mediated Asp transport by glutamate and resulting depletion of meso-diaminopimelate PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • 2-oxoglutarate + L-aspartate --> L-glutamate + oxaloacetate (according to UniProt)
  • Protein family

    Paralogous protein(s)

    Cofactors

  • PLP
  • Structure

  • 1J32 (from Phormidium lapideum, 45% identity, 66% similarity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulation

  • constitutive
  • view in new tab

    Additional information

  • the mRNA is cleaved by RNase III PubMed
  • Biological materials

    Mutant

  • BKE22370 (aspB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT
  • BKK22370 (aspB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT
  • References

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