araQ

ABC transporter for the uptake of -1,5-arabinooligosaccharides, at least up to four L-arabinosyl units (integral membrane protein)

Locus: BSU_28730

Isoelectric point: 9.33

Molecular weight: 31.63 kDa

Protein length: 281 aa

Gene length: 846 bp

Function: uptake of -1,5-arabinooligosaccharides

Product: -1,5-arabinooligosaccharide ABC transporter (integral membrane protein)

Essential: no

Synonyms: yseE

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of carbon sources
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of arabinan/ arabinose/ arabitol
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,939,851 2,940,696

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• MalFG subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 77-266) (according to UniProt)

Structure

• 4TQU (from Sphingomonas sp., 25% identity) PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: araA-araB-araD-araL-araM-araN-araP-araQ-abfA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AraR: repression, PubMed, in AraR regulon

  Regulation

  • induced by arabinose (AraR) PubMed

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-A987 (araQ::erm), available at the NBRP B. subtilis, Japan
• BKE28730 (araQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAACTGAGGACTGTGCCGCA, downstream forward: _UP4_GGTTAATGAAGGAGGAAACG
• BKK28730 (araQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAACTGAGGACTGTGCCGCA, downstream forward: _UP4_GGTTAATGAAGGAGGAAACG

References

Loading