ppaC

inorganic pyrophosphatase

Locus: BSU_40550

Isoelectric point: 4.51

Molecular weight: 33.83 kDa

Protein length: 309 aa

Gene length: 930 bp

Function: recovery of phosphate ions from pyrophosphate

Product: inorganic pyrophosphatase

Essential: yes

E.C.: 3.6.1.1

Synonyms: yybQ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Phosphate metabolism
• Groups of genes → Essential genes

Gene

Coordinates: 4,168,204 4,169,133

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• diphosphate + H₂O --> H⁺ + 2 phosphate (according to UniProt)

Protein family

• PPase class C family (single member, according to UniProt)

Domains

• DHH-DHHA1 domain

Modification

• S-cysteinylation after diamide stress (C158) PubMed
• Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed

Cofactors

• Mn²⁺ PubMed

Structure

• 2HAW (complex with substrate analogue p-nitrophosphate), 1WPM PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ppaC
  Description: PubMed

Biological materials

Mutant

• MGNA-B837 (yybQ::erm), available at the NBRP B. subtilis, Japan

References

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