sacP [Mon Aug 31 2015 13:57:26 GMT+0200 (CEST)]

trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component of the PTS

locus: BSU38050

pI: 7.03

mw: 49.00 kDa

function: sucrose uptake and phosphorylation,
control of SacT activity

product: trigger enzyme: sucrose-specific
phosphotransferase system, EIIBC component

essential: no

ec: 2.7.1.69

synonyms: ipa-49d

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]

syns: ipa-49d

[SW|Categories] containing this gene/protein

• [SW|phosphotransferase systems], [SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|trigger enzyme], [SW|membrane proteins], [SW|phosphoproteins]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|SacT regulon]

The protein

Catalyzed reaction/ biological activity

• Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

Protein family

• [SW|PTS] permease, sucrose permease (Scr) family [Pubmed|10627040]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|sacP]-[protein|search|sacA]-[protein|search|ywdA]'' [Pubmed|3100393]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|8702561]

Regulation

• induced by sucrose ([protein|search|SacT]) [Pubmed|2163394]
• repressed by glucose ([protein|search|CcpA])

Regulatory mechanism

• [protein|search|SacT]: binding to a [SW|RNA switch] results in transcription antitermination [Pubmed|2163394,8702561]
• [protein|search|CcpA]: transcription repression

Biological materials

Expression vector

• for expression, purification of the EIIB domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP429, available in [SW|Stülke] lab
• for expression, purification of the EIIB domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP436, available in [SW|Stülke] lab

References

• 10627040,12107147,3122206,2163394,8702561,2120236,22900538